DOI: 10.1146/annurev.biochem.75.101304.124125 ISSN:

Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry

Benjamin F. Cravatt, Aaron T. Wright, John W. Kozarich
  • Biochemistry

Genome sequencing projects have provided researchers with a complete inventory of the predicted proteins produced by eukaryotic and prokaryotic organisms. Assignment of functions to these proteins represents one of the principal challenges for the field of proteomics. Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function directly in native biological systems on a global scale. Here, we review the basic technology of ABPP, the enzyme classes addressable by this method, and the biological discoveries attributable to its application.

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