DOI: 10.1161/circ.148.suppl_1.16780 ISSN: 0009-7322

Abstract 16780: Single Cell Profiles of Human Heart Failure and Myocardial Diseases

Olof Gidlof, Tomasz Czuba, Neha Pimpalwar, Selvi Celik, Kristina Vukusic, Jakob Lundgren, Entela Bollano, Philipp Rentzsch, Molly Martorella, Erik Linner, Sebastian Albinsson, Jan Boren, Anders Jeppsson, Johan Nilsson, Pradeep Natarajan, Tuuli Lappalainen, Joakim Sandstedt, Malin Levin, Gustav Smith
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Background: Heart failure represents the end-stage of a range of diseases which adversely impact the heart muscle. The extent by which cellular and molecular mechanisms differ between myocardial diseases in heart failure remains incompletely understood.

Methods: We performed isolation and droplet-based RNA-sequencing of 394 247 single nuclei from 103 explanted hearts from transplant and ventricular assist device recipients and 7 unused donor hearts without heart disease. After sample and nuclei level quality control, doublet removal and sample integration; principal component analysis and shared nearest neighbor clustering were used to identify distinct cell populations. Cell type composition and transcriptomic profiles were compared across donor hearts and 10 myocardial diseases (hypertrophic, dilated, ischemic, restrictive, arrythmogenic and non-compaction cardiomyopathy; rheumatic and congenital heart disease; cardiac amyloidosis and graft rejection; n=1-45/group).

Results: We identified and manually annotated 14 distinct cell populations that were present across controls and myocardial diseases, including transcriptionally diverse subgroups of cardiomyocytes, endothelial cells and pericytes. Compared to controls, we observed disease-specific differences in cell populations with regards to composition, heterogeneity, and specific transcriptional profiles.

Conclusions: Our results reveal differences in single cell profiles of individual heart muscle diseases compared to controls without heart disease.

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