DOI: 10.12688/f1000research.184283.1 ISSN: 2046-1402

A Protocol for In Vitro Propagation of Dorema Species (Apiaceae) through Indirect Somatic Embryogenesis and Organogenesis

Dilafruz Jamalova, Mohizar Mirzaolimova, Amir Zarekarizi, Feruza Mustafina, Vasila Sharipova, Khilola Ubaydullayeva, Mukhayyo Kholdorova, Gulsauir Kurbaniyazova, Mirzohid Mirzaolimov, Huseyin Turker, Ziyoviddin Yusupov, Komiljon Tojibaev
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This study describes tissue culture protocols for plantlet regeneration through indirect somatic embryogenesis and indirect organogenesis of Dorema microcarpum Korov. and Dorema sabulosum Litv. (Apiaceae) for the first time. The highest percentage of callusogenesis (90%) was obtained by using 2,4 -D (0.2 and 0.5 mg/l), followed by 2,4 -D (0.5 mg/l) + TDZ (0.5 mg/l) and 2,4-D (0.5 m g/l) + Kin (0.5 mg/l). Additionally, IAA (0.2 mg/l) + BAP (0.5 mg/l) showed the high percentage of callusogenesis (60–70%) for both Dorema species. In D. microcarpum , the highest percentage of callusogenesis was obtained from 2,4-D (0.5 mg/l) + TDZ (0.5 mg/l), while the highest percentage of embryogenesis was achieved with 2,4-D (0.5 mg/l) + Kin (0.5 mg/l). Hypocotyl explants yielded the highest percentage of embryogenesis. For somatic embryo maturation, 2,4-D had to be removed from a nutrient medium. Embryo maturation occurs after the embryo was transferred to a hormone-free medium. For D. sabulosum , morphogenesis occured through indirectly organogenesis, and the most optimal combination for the process of hemogenesis was IAA (0.5 mg/l) + BAP (1.0 mg/l). In vitro propagation of Dorema species in the future will become the basis for continuous year-round propagation of rare, endemic and medicinal species of this genus by biotechnological methods, and will become an alternative to obtaining biologically active compounds of medicinal plants.

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