DOI: 10.1073/pnas.67.1.305 ISSN:

A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate

Alfred G. Gilman
  • Multidisciplinary

A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10 -9 M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

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