In this work, a novel electrochemical aptasensor for the detection of ochratoxin A (OTA) was developed using a three-dimensionally ordered macroporous (3DOM) reduced graphene oxide–Au nanoparticles–molybdenum disulfide (RGO-AuNPs-MoS2) composite material as the sensing interface and Au nanoparticles–carbon-coated ferroferric oxide (AuNPs-Fe3O4@C) composite as the signal amplifier. The 3DOM RGO-AuNPs-MoS2 composite exhibited excellent conductivity and catalytic activity. The 3DOM RGO-AuNPs-MoS2 film was electrodeposited onto the Au electrode surface to immobilize DNA capture probe (CP), which was then hybridized with DNA helper strands (HS) and OTA aptamer (OPT) to form a Y-shaped structure. In the presence of OTA, the OPT was released from the electrode and then combined with AuNPs-Fe3O4@C containing the signal DNA1-metheylene blue (S1-MB) and DNA2-metheylene blue (S2-MB). The current response coming from MB was proportional with the OTA concentration. Under optimal conditions, the linear range of the aptasensor was 1 fg/mL to 0.1 μg/mL, with a detection limit as low as 0.56 fg/mL. The aptasensor was also used to detect OTA in rice and wheat samples, and the results were in agreement with those obtained by liquid chromatography-mass spectrometry (HPLC-MS).

More from our Archive

• DOI: 10.1515/zpch-2023-0252 2023

  Synthesis of doped metal sulfide nanoparticles and their graphene reinforced nanohybrid for Pb(II) detection

  Ghulam Nazik, Muhammad Aadil, Sonia Zulfiqar, Warda Hassan, Abdur Rahman, Sobhy M. Ibrahim, Khalida Naseem, Tahir Ali Sheikh, Muhammad Nadeem Akhtar

• DOI: 10.3390/ijms241310846 2023

  Urinary MicroRNAs as Biomarkers of Urological Cancers: A Systematic Review

  Achille Aveta, Simone Cilio, Roberto Contieri, Gianluca Spena, Luigi Napolitano, Celeste Manfredi, Antonio Franco, Fabio Crocerossa, Clara Cerrato, Matteo Ferro, Francesco Del Giudice, Paolo Verze, Francesco Lasorsa, Andrea Salonia, Rajesh Nair, Jochen Walz, Giuseppe Lucarelli, Savio Domenico Pandolfo

• DOI: 10.3390/molecules28135145 2023

  Cancer Nanomedicine: Emerging Strategies and Therapeutic Potentials

  Manman Xu, Xinpu Han, Hongtai Xiong, Yijie Gao, Bowen Xu, Guanghui Zhu, Jie Li

• DOI: 10.3390/catal13071102 2023

  Recent Progress on Semiconductor Heterogeneous Photocatalysts in Clean Energy Production and Environmental Remediation

  Nahal Goodarzi, Zahra Ashrafi-Peyman, Elahe Khani, Alireza Z. Moshfegh

• DOI: 10.3390/molecules28135282 2023

  Anti-α-Glucosidase, SAR Analysis, and Mechanism Investigation of Indolo[1,2-b]isoquinoline Derivatives

  Mengyue Li, Lin Li, Li Lu, Xuetao Xu, Jinhui Hu, Jin-Bao Peng

• DOI: 10.3390/molecules28135186 2023

  Homogeneous Electrochemical Aptamer Sensor Based on Two-Dimensional Nanocomposite Probe and Nanochannel Modified Electrode for Sensitive Detection of Carcinoembryonic Antigen

  Zhengzheng Yan, Shiyue Zhang, Jiyang Liu, Jun Xing

• DOI: 10.1002/pol.20230273 2023

  Recent advances in covalent organic polymers‐based thin films as memory devices

  Pan‐Ke Zhou, Hongling Yu, Yiping Li, Hong Yu, Qian Chen, Xiong Chen

• DOI: 10.3390/molecules28145407 2023

  Recent Developments and Applications of Microbial Levan, A Versatile Polysaccharide-Based Biopolymer

  Marta Domżał-Kędzia, Monika Ostrowska, Agnieszka Lewińska, Marcin Łukaszewicz

• DOI: 10.3390/chemosensors11070388 2023

  Enzymatic Electrochemical Biosensors for Neurotransmitters Detection: Recent Achievements and Trends

  Zina Fredj, Baljit Singh, Mohamed Bahri, Peiwu Qin, Mohamad Sawan

• DOI: 10.1039/d3cp02529b 2023

  Dynamically changeable terahertz metamaterial absorbers with intelligent switch and high sensitivity and wide and narrow band perfect absorption

  Qian Zhao, Zao Yi, Liang Bian, Huan Liu, Hua Yang, Shubo Cheng, Gongfa Li, Liangcai Zeng, Hailiang Li, Pinghui Wu