DOI: 10.1002/cbdv.202300622 ISSN:

A Homochiral Diphenylalanine Analogue based Mechanoresponsive Hydrogel : An Insight towards its  Wound Healing Efficacy

Anita Dutt Konar, Priyanka Tiwari, Vaibhav Shivhare, Rishabh Ahuja, Naureen Khan, Durgesh Nandan Shukhla, Ankit Mishra, Anindya Basu
  • Molecular Biology
  • Molecular Medicine
  • General Chemistry
  • Biochemistry
  • General Medicine
  • Bioengineering

Deciphering the most promising strategy for the evolution of potential wound‐healing therapeutics is one of the greatest challenging affairs to date. The development of peptide‐based smart scaffolds with innate antimicrobial, anti‐inflammatory, and antioxidant properties is an appealing way out. Aligned to the goal a set of  Hydrogelators I – IV were developed utilizing the concept of chiral orchestration in diphenylalanine fragment, such that the most potent construct with all the bench marks namely mechanoresponsiveness, biocompatibility, consistent antimicrobial and antioxidant properties, could be fished out from the design. Interestingly, our invitro Antifungal and Lipid peroxidation analysis identified the homochiral isomer  (Hydrogelator I), as an ideal candidate for the wound healing experiment. So we proceeded for the invivo  measurements in Wister rats. Indeed the wound images  demonstrated that with increased recovery time, hydrogelator I displayed a significant reduction in the lesion diameter compared to the marketed drug, and negative control. Even the histopathological measurements using H & E staining demonstrated diminished tissue destruction, neutrophil infiltration necrosis, and lymphatic proliferation in the hydrogelators, incomparison to others, backed by invivo lipid peroxidation data. Overall our investigation certifies hydrogelator I as an effective therapeutic for managing the wound healing complication.

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