DOI: 10.12688/f1000research.183125.1 ISSN: 2046-1402

A guide to selecting high-performing antibodies for Syntenin-1 (O00560) for use in western blot, immunoprecipitation, and immunofluorescence

Vera Ruíz Moleón, Charles Alende, Maryam Fothouhi, Sara González Bolívar, Riham Ayoubi, Vincent Francis, Peter S McPherson, Carl Laflamme
Show PDF Cite
Syntenin-1 is the Syndecan-binding protein 1 and a PDZ domain–containing adaptor protein that regulates diverse cellular processes through its interactions with transmembrane receptors, cytoskeletal components, and signaling molecules. Here we have characterized twelve Syntenin-1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.

More from our Archive