DOI: 10.12688/f1000research.182633.1 ISSN: 2046-1402

A guide to selecting high-performing antibodies for DJ-1 (PARK7) (Q99497) for use in western blot, immunoprecipitation, and immunofluorescence

CongYao Zha, Charles Alende, Maryam Fotouhi, Sara González Bolívar, Riham Ayoubi, Vincent Francis, Peter S. McPherson, Carl Laflamme
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DJ-1 is a multifunctional protein that plays a pivotal role in cellular protection against oxidative stress and neurodegeneration. Mutations in the PARK7 gene are associated with early-onset familial Parkinson’s disease. Here we have characterized sixteen DJ-1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.

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