A genetically encodable and fluorogenic photo-crosslinker via photo-induced defluorination acyl fluoride exchangeJielin Fu, Sitong Li, Lijun Deng, Xiaohu Zhao, Zhipeng Yu
- Materials Chemistry
- Metals and Alloys
- Surfaces, Coatings and Films
- General Chemistry
- Ceramics and Composites
- Electronic, Optical and Magnetic Materials
m-TFMAK was developed as a fluorogenic photo-crosslinker, incorporated into proteins via genetic code expansion in living cells. Photo-DAFEx of the encoded m-TFMAK allows proteomic tracking and visualization of protein–protein interactions.