A Distinct Nasal Microbiota Signature in Peritoneal Dialysis Patients
Iman Khan, Sylvia Wu, Anika Hudson, Clayton Hughes, Gabriel Stryjniak, Lars F. Westblade, Michael J. Satlin, Nicholas Tedrow, Anne-Catrin Uhlemann, Colleen Kraft, Darshana M. Dadhania, Jeffrey Silberzweig, Iwijn De Vlaminck, Carol Li, Vesh Srivatana, John Richard Lee- Psychiatry and Mental health
- Neuropsychology and Physiological Psychology
Background
The nasal passages harbor both commensal and pathogenic bacteria and can be associated with infectious complications. The nasal microbiome in peritoneal dialysis (PD) patients, however, has not been well characterized. In this study, we sought to characterize the anterior nasal microbiota in peritoneal dialysis (PD) patients and assess its association with PD peritonitis.
Methods
In this study, we recruited 32 PD patients, 37 kidney transplant (KTx) recipients, and 22 living donor/healthy control (HC) participants and collected their anterior nasal swabs at a single point in time. We followed the PD patients for future development of peritonitis. We performed 16S rRNA gene sequencing of the V4-V5 hypervariable region to determine the nasal microbiota. We compared nasal abundance of common genera among the 3 groups using Wilcoxon rank sum testing with Benjamini-Hochberg adjustment. DESeq2 was also utilized to compare the groups at the amplicon sequence variant levels.
Results
In the entire cohort, the most abundant genera in the nasal microbiota included:
Conclusion
We find a distinct nasal microbiota signature in PD patients compared to KTx recipients and HC participants. Given the potential relationship between the nasal pathogenic bacteria and infectious complications, further studies are needed to define the nasal microbiota associated with these infectious complications and to conduct studies on the manipulation of the nasal microbiota to prevent such complications.