A Dataset of V Subgenome Minisatellites and Molecular Markers from Dasypyrum villosum (L.) P. Candargy for Studying Plants of the Tribe Triticeae Dumortier

DNA repeats make up a significant portion of the genome and are often used for molecular and cytogenetic studies. In some cases, microsatellite (with 1–9 bp monomer length) and minisatellite (with 10–100 bp monomer length) loci are highly polymorphic and suitable for the development of effective DNA markers. The tribe Triticeae includes not only cultivated plants (wheat, rye, barley), but also their wild relatives, which are involved in breeding programs as donors of important agricultural traits. Dasypyrum villosum (2n = 14, VV genome) is an annual cereal plant of the Triticeae tribe, native to the north-eastern Mediterranean region. Successful use of this species for crop improvement, as well as for theoretical understanding of subgenome relationships within the tribe, requires a detailed study of DNA repeats. In this study, a thorough search for minisatellite repeats was performed in the published D. villosum genome. The search results are presented as a dataset, which includes information about minisatellite motifs, their lengths, copy numbers, positions on chromosomes, and array lengths. For most of the identified minisatellite regions, primers were automatically designed. The primers were used for comparative analysis of different subgenomes of the Triticeae tribe by electronic PCR (e-PCR). Some primers revealed clear polymorphism among subgenomes and were selected for conventional PCR experiments and fragment analysis, demonstrating the practical utility of the presented dataset. Thus, the dataset can be useful for researchers and practicing breeders who study D. villosum and other plants from the Triticeae tribe.