T. Anitha, Archana K. Sanketh, Natarajan Kalavathy, Mitha M. Shetty, P. Roshan Kumar, Anuradha Venkataramani

A comparative evaluation of antimicrobial property of traditional and three alternative disinfectants on irreversible hydrocolloid impressions: An in vitro study

  • General Dentistry
  • Oral Surgery
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Aim:The aim was to compare the efficacy of various herbal disinfectants on irreversible hydrocolloid impressions and to investigate the effectiveness of three herbal disinfectants and a chemical disinfectant against particular pathogens.Settings and Design:In vitro-a comparative study.Materials and Methods:The following methodology was followed to achieve the objectives. Four maxillary impressions were made for each selected patient with irreversible hydrocolloid impression material. The predisinfection swabs were taken from impression sites of teeth 17, 13, 27, and 23 (FDI system of tooth numbering). The impressions were immersed in all four different disinfectants such as 2% glutaraldehyde, Aloe vera solution, 50% neem oil, and apple vinegar solution, then the postdisinfection swabs were taken from the same sites 17,13,27,23 and then cultured onto sheep blood agar and examined for growth, and colony forming units (CFUs) ofStreptococcus viridans,Streptococcus mutans,Streptococcus sanguis, andActinomyces viscosus. The comparative analysis was done for the predisinfection and postdisinfection values in each study group.Statistical Analysis Used:Descriptive analysis, Kruskal Wallis test, Mann Whitney post hoc test, Wilcoxon signed rank test.Results:The results revealed that the mean CFUs ofS. viridans,S. mutans,S. sanguis, andA. viscosusduring postdisinfection samples were statistically significant when compared to predisinfection samples. Multiple comparison of the mean CFUs of all 4 microorganisms in the control group and in 50% Neem oil group was significantly lesser compared toA. veraand Apple Vinegar group.Conclusion:CFUs ofS. viridans,S. mutans,S. sanguis, andA. viscosussignificantly decreased in the 50% neem oil group as well as the control group. As a result, 50% Neem oil was a viable option for disinfecting alginate impressions.

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