424. EXPRESSION OF KCNB1 AND ITS RELATION TO TUMOR PROGRESSION IN ESOPHAGEAL CANCER
Atsuki Ota, Atsushi Shiozaki, Keiji Nishibeppu, Jun Kiuchi, Takuma Ohashi, Hiroki Shimizu, Tomohiro Arita, Yusuke Yamamoto, Hirotaka Konishi, Ryo Morimura, Yoshiaki Kuriu, Hisashi Ikoma, Takeshi Kubota, Hitoshi Fujiwara, Eigo Otsuji- Gastroenterology
- General Medicine
Abstract
Background
KCNB1 is a member of voltage-gated potassium channels, which mediate transmembrane potassium transport. Previous studies have elucidated the roles of and the mechanisms by which KCNB1 is activated in various cancer type. However, the role of KCNB1 in esophageal cancer remains poorly understood. In the present study, we analyzed the relationship between KCNB1 expression and tumor progression in esophageal cancer.
Methods
Knockdown (KD) experiments were performed on human esophageal cancer cell lines using KCNB1 small interfering RNA. 129 primary tissue samples from esophageal cancer patients were examined immunohistochemistry (IHC), and its relationship with clinicopathological factors and prognosis were examined.
Results
KCNB1-KD suppressed the proliferation, migration and invasion of cells and enhanced apoptosis. Cell cycle analysis showed that KCNB1-KD suppressed the progression of G2/M phase. Survival analysis showed significantly poorer 5-year relapse-free survival (RFS) in the KCNB1 high expression group by IHC (high vs low; 28.5% vs 58.1%, p = 0.0016). The multivariate analysis identified the high expression of KCNB1 as an independent prognostic factor for 5-year RFS in esophageal cancer patients (p = 0.0197).
Conclusion
The present study may contribute to the identification of KCNB1 as a mediator in tumor progression, with it eventually being a promising prognostic biomarker or a novel therapeutic target of esophageal cancer.