#138 : Effect of Endometriosis on Human ICSI, and Endometriosis Follicle Fluid on Mouse Oocyte Maturation and Mitochondria
Ida Bagus Praja Putra Adnyana, Jaqueline Sudiman, Ida Bagus Putra Adnyana, Tjokorda Chandragiram, Ni Nyoman Ayu Dewi, Anom Suardika, I Gusti Widianti, Ketut Suwiyoga, I Gusti Ngurah Bagus Surya Udayana- General Medicine
Background and Aims: Endometriosis is the common cause of infertility. Assisted Reproductive Technology has become a promising choice for women with endometriosis, however, there is no consensus concerning the impact of endometriosis on the success rate. Our study was aimed to assess the impact of endometriosis on ICSI and to investigate the effect of endometriosis follicle fluid (FF) on mouse oocytes maturation and mitochondrial activity.
Methods: Hormonal stimulation, embryo quality and success rate from moderate-severe and minimal-mild endometriosis were compared with tubal infertility patients as controls. Then animal experiments were carried out involving the co-culturing of mouse oocytes with FF from moderate-severe endometriosis patients to assess mitochondrial dye, oocyte maturation, and protein levels of cytochrome C and cyclin B1 using ELISA.
Results: The number of follicles, mature oocytes, and fertilization rates were significantly lower in moderate-severe endometriosis group compared to control and minimal-mild endometriosis groups. This led to significantly decreased pregnancy rates in moderate-severe endometriosis group compared to minimal-mild endometriosis and control groups [26/72 (36.1%); 27/47 (57.4%); 85/152 (55.9%), p<0.05, respectively]. Implantation rates in moderate-severe endometriosis group decreased significantly compared to control group [31/166 (18.7%) vs 113/392 (28.8%), OR 1.76; 95% CI 1.14 to 2.79; p<0.05). Mitochondrial dye was significantly lower in mouse oocytes cultured with endometriosis FF compared to control and oocytes cultured with tubal factor FF. Cyclin B1 protein level was lower in oocytes cultured with endometriosis FF compared to control and oocytes cultured with tubal factor FF. There was no difference in cytochrome C protein level among groups.
Conclusions: Our results showed that moderate-severe endometriosis is associated with reduced quality of oocyte and embryo and leads to lower success rates of ICSI. This research also showed FF could impair mouse oocytes nuclear maturation which may be related to reduced cyclin B1 level and decrease activity of mitochondria.