#103 : The Clinical Outcomes of Lasered Assisted Hatching on Single Frozen-Thawed Blastocyst Cycle: A Retrospective, Observational Study

Background and Aims: The ultimate goal in assisted reproductive field is to achieve a pregnancy. For embryo to implant, embryo must be hatched from zona pellucida. Therefore, pregnancy began. Lasered assisted hatching (LAH) has been proposed as one of the interventions to increase the success rate, yet the efficacy remains uncertain. Hence, the aim of this study was to evaluate the effect of LAH toward clinical outcomes including clinical pregnancy, miscarriage, and ectopic pregnancy rate on single frozen-thawed blastocyst cycle.

Method: A total of 1553 single frozen-thawed blastocyst cycles (LAH, n=116; non-LAH, n=1417) with patient’s mean age of 35 ± 0.06 were recruited from 2018 to 2022. The exclusion criteria were as follows: (a) gamete/embryo donation cycle and (b) PGT cases. Assisted hatching procedure was performed on LAH group with 1.46-[Formula: see text]m noncontact diode laser (LYKOS^Ⓡ Laser) prior to embryo transfer.

Result: The clinical pregnancy, miscarriage and ectopic pregnancy rates for LAH and non-LAH groups were 44.8% versus 48.7%, p = 0.35, 17.3% versus 16.2%, p = 0.86 and 0% versus 0.7%, respectively. No statistically significant difference was found on these clinical outcomes.

Conclusion: This study demonstrated the use of LAH has no impact on the clinical outcomes and did not increase miscarriage and ectopic pregnancy. Therefore, it can be a useful tool for patients with recurrent implantation failure or embryo with thick zona pellucida.