DOI: 10.70879/2umuegae2 ISSN:

Ảnh hưởng của nhiệt độ và môi trường nuôi cấy đến sinh trưởng hệ sợi nấm Linh chi cổ cò (Ganoderma neo-japonicum Imazeki) thu nhận tại tỉnh Khánh Hòa

An Khúc Thị
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<p>Ganoderma neo-japonicum is a valuable medicinal mushroom with significant potential for pharmaceutical and biotechnological applications. However, optimal conditions for its mycelial cultivation remain limited. This study evaluated the effects of incubation temperature (15, 20, 25, 30, and 35°C) and culture media on the mycelial growth of a G. neo-japonicum strain collected from Khanh Hoa Province, Vietnam. Five media were compared: potato dextrose agar (PDA), PDA supplemented with rice bran (PRB) or yeast extract (PYE) or mineral salts (PKM), and malt extract medium (ME). Colony diameter, growth rate, and morphology were recorded over 14 days. Temperature significantly affected mycelial growth, with active expansion occurring exclusively at 25 and 30°C. Although 30°C promoted faster initial growth, 25°C yielded more stable development, producing dense, uniformly white mycelia and the largest final colony diameter. Prolonged incubation at 30°C resulted in reduced mycelial density and yellowish pigmentation. Culture media also markedly influenced development: PYE supported the fastest early expansion, PRB achieved the greatest overall growth and largest final colony diameter, whereas ME resulted in the slowest growth. In conclusion, incubation at 25°C on PDA supplemented with rice bran provides the most suitable conditions for laboratory cultivation of G. neo-japonicum, providing a reliable basis for future spawn production and domestication.</p>

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