УДK:616.96:578.842.94 - 036.22 -079.4 TAJRIBA HAYVONLARIDA BRUTSELLYOZ QO‘ZG‘ATUVCHILARIGA QARSHI IJOBIY STANDART POLIVALENT ZARDOBLARNI OLISH

The article presents the results of experimental studies aimed at obtaining a standard polyvalent serum against brucellosis pathogens to improve early diagnosis and serological detection methods. The experiment was conducted on 12 rabbits divided into four groups, which were immunized with the live B. abortus 19 vaccine and inactivated Brucella culture suspensions at various concentrations following different immunization schemes. The obtained sera were analyzed for total protein, albumin, globulin, IgA, IgM, and IgG levels, and the dynamics of their changes were assessed during immune response formation. The results showed that immunization with the live attenuated B. abortus 19 vaccine produced a more stable and long-lasting immune response, while the use of Incomplete Freund’s Adjuvant (IFA) enhanced immunoglobulin production. Based on the obtained data, scientific and practical foundations were developed for preparing a national standard serum against brucellosis. The application of this serum will enable the standardization of serological diagnostics, quality control of diagnostic preparations, and ensure comparability of research results at the international level.. 111