THE INFLUENCE OF +Gz ACCELERATION ON Th1 AND Th2 POLARIZATION OF THE IMMUNE SYSTEM IN RATS

<p>Introduction/Aim. +Gz acceleration is unique dynamic stress to organism. Development of this occurrence depends not only to magnitude of acceleration but to onset rate and duration too (1). The most of former researches pointed to +Gz stress as significant stress to basic physiological mechanisms like cardiovascular and respiratory system. The aim of our study was to examine responses of neuroendocrine and immune system to +Gz stress. Method. Examination was performed in two inbred strains of rats Albino Oxford (AO) and Dark Agouti (DA). AO, n=45 and DA, n=60, male, 9-11 weeks old. Experimental animals were subjected to the Test of linear increase of +Gz acceleration (peak 7G, onset rate 0.1 g/s) at the Institute of aviation medicine of Military Medical Academy. Before examination, animals fasted 24 hours. Control group were rats which did not subjected to +Gz acceleration. After the animals were sacrificed we punctured blood from abdominal aorta to examine plasma and serum and extirpated thymus and spleen to be examined, too. In wish to examine effects of +Gz stress to polarization of Th immune response we determined concentrations of cytokines (IFN-ã and IL-4) in the culture of spleen cells. Results. The level of cytokines in the culture of spleen cells stimulated with Con A, isolated from animals (experimental and control group) 3 and 24 hour after +Gz exposure and level of cytokines determined in supernatants, showed statistical significance as it presented on the graphics. Conclusion. According to our research obtained on animals we can conclude that +Gz acceleration in the early period (after 3 hours) decreased IFN-ã in the culture of spleen cells. AO rats showed difference compared to DA rats in reaction to +Gz stress. AO rats had increase in IL-4 in the culture of spleen cells and higher decrease of IFN-ã and emphasized polarization of Th1/Th2 in course to Th2. After 24 hours of exposure to +Gz stress we observed increase of IFN-ã (DA) and increase of IL-4 (AO).</p>