Global Mapping of the Yeast Genetic Interaction Network
Amy Hin Yan Tong, Guillaume Lesage, Gary D. Bader, Huiming Ding, Hong Xu, Xiaofeng Xin, James Young, Gabriel F. Berriz, Renee L. Brost, Michael Chang, YiQun Chen, Xin Cheng, Gordon Chua, Helena Friesen, Debra S. Goldberg, Jennifer Haynes, Christine Humphries, Grace He, Shamiza Hussein, Lizhu Ke, Nevan Krogan, Zhijian Li, Joshua N. Levinson, Hong Lu, Patrice Ménard, Christella Munyana, Ainslie B. Parsons, Owen Ryan, Raffi Tonikian, Tania Roberts, Anne-Marie Sdicu, Jesse Shapiro, Bilal Sheikh, Bernhard Suter, Sharyl L. Wong, Lan V. Zhang, Hongwei Zhu, Christopher G. Burd, Sean Munro, Chris Sander, Jasper Rine, Jack Greenblatt, Matthias Peter, Anthony Bretscher, Graham Bell, Frederick P. Roth, Grant W. Brown, Brenda Andrews, Howard Bussey, Charles Boone- Multidisciplinary
A genetic interaction network containing ∼1000 genes and ∼4000 interactions was mapped by crossing mutations in 132 different query genes into a set of ∼4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.