GLA-loaded Liposome Albumin Particles-mediated p53 Targets Cervical Cancer Cell Transplant Tumors
Yanzi Du, Chengyun LiuBackground
p53 is a phosphorylated glycoprotein whose expression is abnormally elevated in various human tumors. p53 also regulates a variety of tumor cells, but research on cervical cancer has not yet been reported.
Purpose
This study intends to investigate the role of targeting p53 in cervical cancer.
Methods
Electron microscopy was used to assess the size of protein particles, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) was used to detect the OD value of nanocomposites at different concentrations using a UV spectrophotometer, MTT and cell cloning were used to detect cell proliferation expression ability, and reverse transcriptase polymerase chain reaction (RT-PCR) to detect p53 expression. Immunofluorescence measured nuclear factor kappa B (NF-κB) expression in cells, and western blotting assessed the relative expression of p53 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) proteins. The detection mechanism was further explored through in vivo studies.
Results
Glutaraldehyde (GLA)-loaded liposomal albumin particles showed a positive zeta value, indicating that the CS coating process of GLA was successful. Our data show that the experimental group showed a concentration-dose-dependent growth relationship, indicating that GLA-loaded liposome albumin particles have better antitumor activity in cervical cancer cell lines. Compared with most GLA-loaded liposomal albumin particle groups, P53 expression was inhibited, and the expression of P53 was downregulated in GLA-loaded liposomal albumin particle cervical cancer. In vivo results showed that the measured tumor volume decreased after treatment with GLA-loaded liposomal albumin particles, and mice treated with GLA-loaded liposomal albumin particles had longer survival times. Induction of NF-κB in tumors may be a powerful strategy allowing GLA-loaded liposomal albumin particles to avoid apoptosis and possibly after cytotoxic chemotherapy.
Conclusion
This study demonstrates that GLA-loaded liposomal albumin particles may participate in the development and progression of human cervical cancer cells by targeted regulation of NF-κB activity.