DOI: 10.1093/bbb/zbad175 ISSN: 1347-6947
Extracellular vesicles of Weizmannia coagulans lilac-01 reduced cell death of primary microglia and increased mitochondrial content in dermal fibroblasts in vitro
Kimiko Minamida, Toshio Taira, Masato Sasaki, Ohki Higuchi, Xian-Ying Meng, Yoichi Kamagata, Kazunori Miwa- Organic Chemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- General Medicine
- Biochemistry
- Analytical Chemistry
- Biotechnology
ABSTRACT
We investigated the properties of extracellular vesicles from the probiotic Weizmannia coagulans lilac-01 (Lilac-01EVs). The phospholipids in the Lilac-01EV membrane were phosphatidylglycerol and mitochondria-specific cardiolipin. We found that applying Lilac-01EVs to primary rat microglia in vitro resulted in a reduction in primary microglial cell death (P < .05). Lilac-01EVs, which contain cardiolipin and phosphatidylglycerol, may have the potential to inhibit cell death in primary microglia. The addition of Lilac-01EVs to senescent human dermal fibroblasts suggested that Lilac-01 EVs increase the mitochondrial content without affecting their membrane potential in these cells.