DNA adducts form in mouse lung and liver after oral naphthalene exposure

Abstract

Naphthalene is a ubiquitous combustion product and environmental contaminant with known human exposure. Chronic exposure to naphthalene vapor leads to respiratory tumor formation in rodents. Naphthalene forms DNA adducts (precursors to genotoxicity) in tissue explants but it is unclear if this occurs in vivo. Wild-type C57BL/6 mice were orally exposed to 50 mg/kg 14C-naphthalene. Naphthalene-DNA adducts were detected by accelerator mass spectrometry at 2-72 hours post-exposure in both lung and liver, with decreasing abundance over time. Adducts persisted even at 72 hours after exposure, which indicates possible evasion of DNA repair and potential to contribute to mutagenesis.