DOI: 10.1039/d3nr03344a ISSN:
Considerable slowdown of short DNA fragment translocation across a protein nanopore using pH-induced generation of enthalpic traps inside the permeation pathway
Loredana Mereuta, Alina Asandei, Ioan Andricioaei, Jonggwan Park, Yoonkyung Park, Tudor Luchian- General Materials Science
At acidic vs. neutral pH, the α-HL nanopore facillitates electrostatic anchoring of captured ssDNAs, leading to a dramatic increase of the translocation time. This effect is side invariant and sensitive to the ionic strength and ssDNA contour length.